annexin v fitc propidium iodide pi dual staining kit  (Keygen Biotech)

Journal: Scientific Reports

Article Title: The combined inhibitory effect of butaselen and decitabine against lung cancer cells

doi: 10.1038/s41598-026-45054-7

Figure Lengend Snippet: BS combined with DAC showed enhanced effects in inhibiting the survival of lung cancer cells. A549 and H1299 cells were treated with BS, DAC, or BS + DAC (3:1 for A549 [BS-5 μM, DAC-1.67 μM] and 2:1 for H1299 [BS-5 μM, DAC-2.5 μM]) for 48 h, followed by Annexin V-PI staining. The results of total apoptosis (including both early and late apoptotic populations) are shown as mean ± SD. * P < 0.05, *** P < 0.001, **** P < 0.0001, compared with each indicated group by one-way ANOVA followed by Tukey’s post hoc test analysis ( n = 3).

Article Snippet: The cells were collected, and the apoptosis assay was conducted using the Annexin V-FITC/ propidium iodide (PI) Dual Staining Kit (KeyGEN) as instructed by the manufacturer and described previously .

Techniques: Staining

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway

doi: 10.12659/MSM.928403

Figure Lengend Snippet: Piperine induces the apoptosis of SNU-16 cells. ( A ) SNU-16 cells were exposed to piperine (50, 100, 150 μM) for 24 h. Percentage of cell apoptosis was determined by annexin V/PI staining and flow cytometry. ( B ) The apoptotic rates of SNU-16 cells induced by piperine. Data are presented as the mean±SD of at least 3 independent experiments, * P <0.05, ** P <0.01 and *** P <0.001 compared with control cells.

Article Snippet: After that, the cell pellet was resuspended in 500 μl binding buffer and the apoptosis rate was examined by annexin V-FITC/PI (fluorescein isothiocyanate/propidium iodide) dual staining apoptosis detection kit (Nanjing KeyGEN Biotech.

Techniques: Staining, Flow Cytometry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway

doi: 10.12659/MSM.928403

Figure Lengend Snippet: The efficacy of Akt activator (SC79) on piperine-induced apoptosis. SNU-16 cells were determined after treatment with piperine for 24 h in the presence or absence of SC79 (10 μM). ( A ) Apoptosis detection with annexin V/PI double staining in different groups by flow cytometry. ( B ) Column bar graph of mean cell florescence for early apoptotic, late apoptotic, and total apoptotic cells. ( C ) Expression of pAkt, Akt, cleaved caspase3, and cleaved PARP proteins in the presence or absence of SC79. ( D ) Quantification of pAkt, Akt, cleaved caspase3, and cleaved PARP proteins. ( E ) Expression of Bcl-2 and Bax proteins after piperine and/or SC79 treatment. ( F ) Quantification of Bcl-2 and Bax proteins. The Bcl-2/Bax ratio was significantly upregulated in the presence of SC79. Data are presented as the mean±SD of at least 3 independent experiments, * P <0.05, ** P <0.01 and *** P <0.001 compared with control cells; # P <0.05, ## P <0.01 and ### P <0.001 compared with the piperine alone group.

Article Snippet: After that, the cell pellet was resuspended in 500 μl binding buffer and the apoptosis rate was examined by annexin V-FITC/PI (fluorescein isothiocyanate/propidium iodide) dual staining apoptosis detection kit (Nanjing KeyGEN Biotech.

Techniques: Double Staining, Flow Cytometry, Expressing